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KMID : 0545119980080040416
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 4 p.416 ~ p.421
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Involvement of Electrostatic Interactions between the Components of Toluene Dioxygenase from Pseudomonas putida F1
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Lee Kyoung
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Abstract
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A carboxyl group modifier, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was used to study the interactions between three components of toluene dioxygenase (TDO) from Pseudomonas putida F1. Ferredoxin_TOL activity was increased by the treatment with EDC; however, the activity was rapidly declined in the prolonged incubation. In covalent cross-linking experiments with EDC, Ferredoxin_TOL made a one-to-one complex with Reductase_TOL or the large subunit of ISP_TOL. These results provide evidence for the involvement of electrostatic interactions in the TDO electron transfer system.
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KEYWORD
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